Abstract
Background and Purpose
We previously showed that mice with reduced levels of the transforming growth factor-beta
(TGF-β) co-receptor endoglin (Eng+/− mice) develop less fibrosis and vascular damage after kidney irradiation than their
wild type (Eng+/+ mice) littermates; however, the underlying mechanism was unclear. Results from current
studies suggest that this occurs via modulation of the radiation-induced inflammatory
response.
Materials and methods
Kidneys of Eng+/+ and Eng+/− mice were irradiated with 16 Gy. Mice were sacrificed at 20 weeks after irradiation and gene expression and protein levels were analyzed.
Results
Kidney irradiation triggered the infiltration of macrophages in both Eng+/+ and Eng+/− mice, however, levels of macrophage-produced cytokines interleukin 1 beta (Il1b) and interleukin 6 (Il6) were reduced in irradiated Eng+/− compared to Eng+/+ mice. Double immuno-stainings confirmed that IL-6 was produced by macrophages, whereas
IL-1β was mainly detected in other cell types. Accordingly, inflammatory cell precursors
derived from the bone marrow of Eng+/− mice showed impaired ability to express Il1b and Il6 compared to wild type mice.
Conclusions
Endoglin promotes kidney inflammation after irradiation by regulating macrophage infiltration
and interleukin production, thereby promoting pathogenic changes after radiation exposure.
Keywords
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Article info
Publication history
Published online: October 01, 2012
Accepted:
August 17,
2012
Received in revised form:
August 16,
2012
Received:
March 20,
2012
Identification
Copyright
© 2012 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
