Radiotherapy & Oncology
Volume 95, Issue 1 , Pages 66-72 , April 2010

Relative biological effectiveness of pulsed and continuous 20MeV protons for micronucleus induction in 3D human reconstructed skin tissue

  • Thomas E. Schmid

      Affiliations

    • Department of Radiation Oncology, Technische Universitaet Muenchen, Germany
    • Corresponding Author InformationCorresponding author at: Klinikum rechts der Isar der Technischen Universität München, Klinik für Strahlentherapie und radiologische Onkologie Ismaningerstr. 22, 81675 München, Germany.
  • ,
  • Günther Dollinger

      Affiliations

    • Institute for Applied Physics and Metrology, Universitaet der Bundeswehr Muenchen, Neubiberg, Germany
  • ,
  • Volker Hable

      Affiliations

    • Institute for Applied Physics and Metrology, Universitaet der Bundeswehr Muenchen, Neubiberg, Germany
  • ,
  • Christoph Greubel

      Affiliations

    • Institute for Applied Physics and Metrology, Universitaet der Bundeswehr Muenchen, Neubiberg, Germany
  • ,
  • Olga Zlobinskaya

      Affiliations

    • Department of Radiation Oncology, Technische Universitaet Muenchen, Germany
  • ,
  • Dörte Michalski

      Affiliations

    • Department of Radiation Oncology, Technische Universitaet Muenchen, Germany
  • ,
  • Michael Molls

      Affiliations

    • Department of Radiation Oncology, Technische Universitaet Muenchen, Germany
  • ,
  • Barbara Röper

      Affiliations

    • Department of Radiation Oncology, Technische Universitaet Muenchen, Germany

Received 22 December 2009 ,Revised 25 February 2010 ,Accepted 7 March 2010.

References 

  1. Martin M. Laser accelerated radiotherapy: is it on its way to the clinic?. J Natl Cancer Inst. 2009;101:450–451
  2. Luo W, Fourkal E, Li J, Ma CM. Particle selection and beam collimation system for laser-accelerated proton beam therapy. Med Phys. 2005;32:794–806
  3. Salamin YI, Harman Z, Keitel CH. Direct high-power laser acceleration of ions for medical applications. Phys Rev Lett. 2008;100:155004
  4. Henig A, Kiefer D, Markey K, et al. Enhanced laser-driven ion acceleration in the relativistic transparency regime. Phys Rev Lett. 2009;103:045002
  5. Yogo A, Daido H, Bulanov SV, et al. Laser ion acceleration via control of the near-critical density target. Phys Rev. 2008;77:016401
  6. Chiu C, Fomytskyi M, Grigsby F, Raischel F, Downer MC, Tajima T. Laser electron accelerators for radiation medicine: a feasibility study. Med Phys. 2004;31:2042–2052
  7. Fan J, Luo W, Fourkal E, et al. Shielding design for a laser-accelerated proton therapy system. Phys Med Biol. 2007;52:3913–3930
  8. Henig A, Kiefer D, Geissler M, et al. Laser-driven shock acceleration of ion beams from spherical mass-limited targets. Phys Rev Lett. 2009;102:095002
  9. Kreipl MS, Friedland W, Paretzke HG. Interaction of ion tracks in spatial and temporal proximity. Radiat Environ Biophys. 2009;48:349–359
  10. Kreipl MS, Friedland W, Paretzke HG. Time- and space-resolved Monte Carlo study of water radiolysis for photon, electron and ion irradiation. Radiat Environ Biophys. 2009;48:11–20
  11. Plante I, Filali-Mouhim A, Jay-Gerin J. SimulRad: a Java interface for a Monte-Carlo simulation code to visualize in 3D the early stages of water radiolysis. Radiat Phys Chem. 2005;72:173–180
  12. Belyakov OV, Mitchell SA, Parikh D, et al. Biological effects in unirradiated human tissue induced by radiation damage up to 1mm away. PNAS. 2005;102:14203–14208
  13. Sedelnikova OA, Nakamura A, Kovalchuk O, et al. DNA double-strand breaks form in bystander cells after microbeam irradiation of three-dimensional human tissue models. Cancer Res. 2007;67:4295–4302
  14. Tillman C, Grafstrom G, Jonsson AC, et al. Survival of mammalian cells exposed to ultrahigh dose rates from a laser-produced plasma X-ray source. Radiology. 1999;213:860–865
  15. Dollinger G, Bergmaier A, Hable V, et al. Nanosecond pulsed proton microbeam. Nucl Instr Meth Phys Res B. 2009;267:2008–2012
  16. Schmid TE, Dollinger G, Hauptner A, et al. No evidence for a different RBE between pulsed and continuous 20MeV protons. Radiat Res. 2009;172:567–574
  17. Curren RD, Mun GC, Gibson DP, Aardema MJ. Development of a method for assessing micronucleus induction in a 3D human skin model (EpiDerm). Mutat Res. 2006;607:192–204
  18. Mun GC, Aardema MJ, Hu T, et al. Further development of the EpiDerm 3D reconstructed human skin micronucleus (RSMN) assay. Mutat Res. 2009;673:92–99
  19. Hu T, Kaluzhny Y, Mun GC, et al. Intralaboratory and interlaboratory evaluation of the EpiDerm 3D human reconstructed skin micronucleus (RSMN) assay. Mutat Res. 2009;673:100–108
  20. Hauptner A, Dietzel S, Drexler GA, et al. Microirradiation of cells with energetic heavy ions. Radiat Environ Biophys. 2004;42:237–245
  21. Hauptner A, Krucken R, Greubel C, et al. DNA-repair protein distribution along the tracks of energetic ions. Radiat Prot Dosim. 2006;122:147–149
  22. Greubel C, Hable V, Drexler GA, et al. Quantitative analysis of DNA-damage response factors after sequential ion microirradiation. Radiat Environ Biophys. 2008;47:415–422
  23. Ziegler, J, Biersack, J. The stopping and range of ions in matter. SRIM2003 2003. Available from: http://www.srim.org.
  24. Jagetia GC, Aruna R. Correlation of micronuclei-induction with the cell survival in HeLa cells treated with a base analogue, azidothymidine (AZT) before exposure to different doses of gamma-radiation. Toxicol Lett. 2003;139:33–43
  25. International Commission on Radiological Protection (ICRP). Relative biological effectiveness (RBE), quality factor (Q), and radiation weighting factor (wR). Publication 92. New York: Elsevier Inc. Pergamon Press; 2003.
  26. Nias AHW, Swallow AJ, Keene JP, Hodgson BW. Survival of HeLa cells from 10 nanosecond pulses of electrons. Int J Radiat Biol. 1970;17:595–598
  27. Epp ER, Weiss H, Djordjevic B, Santomasso A. The radiosensitivity of cultured mammalian cells exposed to single high intensity pulses of electrons in various concentrations of oxygen. Radiat Res. 1972;52:324–332
  28. Tillman C, Grafström G, Jönnon AC, et al. Survival of mammalian cells exposed to ultrahigh dose rates from a laser-produced plasma X-ray source. Radiology. 1999;213:860–865
  29. Shinohara K, Nakano H, Miyazaki N, Tago M, Kodama R. Effects of single pulse (⩽1ps) X-rays from laser-produced plasmas on mammalian cells. J Radiat Res. 2004;45:509–514
  30. Slonina D, Spekl K, Panteleeva A, Brankovic K, Hoinkis C, Dörr W. Induction of micronuclei in human fibroblasts and keratinocytes by 25kV X-rays. Radiat Environ Biophys. 2003;42:55–61

PII: S0167-8140(10)00162-3

doi: 10.1016/j.radonc.2010.03.010

Radiotherapy & Oncology
Volume 95, Issue 1 , Pages 66-72 , April 2010