Radiotherapy & Oncology
Volume 94, Issue 1 , Pages 110-116 , January 2010

Radiocontrast media affect radiation-induced DNA damage repair in vitro and in vivo by affecting Akt signalling

  • Mahmoud Toulany

      Affiliations

    • Department of Radiation Oncology, University of Tuebingen, Germany
  • ,
  • Rainer Kehlbach

      Affiliations

    • Department of Diagnostic Radiology, University of Tuebingen, Germany
  • ,
  • H. Peter Rodemann

      Affiliations

    • Department of Radiation Oncology, University of Tuebingen, Germany
    • Corresponding Author InformationCorresponding authors. Addresses: Division of Radiobiology and Molecular Environmental Research, Department of Radiation Oncology, Eberhard-Karls University Tuebingen, Roentgenweg 11, 72076 Tuebingen, Germany (H.Peter Rodemann); Department of Medical Genetics, School of Medical Sciences, Tarbiat Modares University, Tehran, P.O. Box 14115-111, Iran (H. Mozdarani).
  • ,
  • Hossein Mozdarani

      Affiliations

    • Department of Medical Genetics, Tarbiat Modares University, Tehran, Iran
    • Corresponding Author InformationCorresponding authors. Addresses: Division of Radiobiology and Molecular Environmental Research, Department of Radiation Oncology, Eberhard-Karls University Tuebingen, Roentgenweg 11, 72076 Tuebingen, Germany (H.Peter Rodemann); Department of Medical Genetics, School of Medical Sciences, Tarbiat Modares University, Tehran, P.O. Box 14115-111, Iran (H. Mozdarani).

Received 4 June 2009 ,Revised 19 October 2009 ,Accepted 16 November 2009.

References 

  1. Beddar AS, Briere TM, Balter P, et al. 4D-CT imaging with synchronized intravenous contrast injection to improve delineation of liver tumors for treatment planning. Radiother Oncol. 2008;87:445–448
  2. Shibamoto Y, Naruse A, Fukuma H, Ayakawa S, Sugie C, Tomita N. Influence of contrast materials on dose calculation in radiotherapy planning using computed tomography for tumors at various anatomical regions: a prospective study. Radiother Oncol. 2007;84:52–55
  3. Girinsky T, Specht L, Ghalibafian M, et al. The conundrum of Hodgkin lymphoma nodes: to be or not to be included in the involved node radiation fields. The EORTC-GELA lymphoma group guidelines. Radiother Oncol. 2008;88:202–210
  4. Norman A, Cochran ST, Sayre JW. Meta-analysis of increases in micronuclei in peripheral blood lymphocytes after angiography or excretory urography. Radiat Res. 2001;155:740–743
  5. Mozdarani H, Fadaei S. Similar cytogenetic effects of sodium-meglumine diatrizoate and sodium-meglumine ioxithalamate in lymphocytes of patients undergoing brain CT scan. Toxicol Lett. 1998;98:25–30
  6. Matsubara S, Katoh T, Kubota N, Yoshino N, Sasaki T, Sasaki MS. The effects of X-ray energy and an iodine-based contrast agent on chromosome aberrations. Radiat Res. 1994;137:231–237
  7. Matsubara S, Kong ZS, Omura M, et al. The effect of iodine-based contrast agents on the levels of radiation-induced chromosomal aberrations. Radiat Res. 1997;147:263–268
  8. Cochran ST, Bomyea K. Trends in adverse events from iodinated contrast media. Acad Radiol. 2002;9:S65–S68
  9. Cochran ST. Anaphylactoid reactions to radiocontrast media. Curr Allergy Asthma Rep. 2005;5:28–31
  10. Andreucci M, Fuiano G, Presta P, et al. Radiocontrast media cause dephosphorylation of Akt and downstream signaling targets in human renal proximal tubular cells. Biochem Pharmacol. 2006;72:1334–1342
  11. Itoh Y, Yano T, Sendo T, et al. Involvement of de novo ceramide synthesis in radiocontrast-induced renal tubular cell injury. Kidney Int. 2006;69:288–297
  12. Yano T, Itoh Y, Sendo T, Kubota T, Oishi R. Cyclic AMP reverses radiocontrast media-induced apoptosis in LLC-PK1 cells by activating A kinase/PI3 kinase. Kidney Int. 2003;64:2052–2063
  13. Fayard E, Tintignac LA, Baudry A, Hemmings BA. Protein kinase B/Akt at a glance. J Cell Sci. 2005;118:5675–5678
  14. Mukohara T, Kudoh S, Matsuura K, et al. Activated Akt expression has significant correlation with EGFR and TGF-alpha expressions in stage I NSCLC. Anticancer Res. 2004;24:11–17
  15. Toulany M, Dittmann K, Baumann M, Rodemann HP. Radiosensitization of Ras-mutated human tumor cells in vitro by the specific EGF receptor antagonist BIBX1382BS. Radiother Oncol. 2005;74:117–129
  16. Toulany M, Kasten-Pisula U, Brammer I, et al. Blockage of epidermal growth factor receptor-phosphatidylinositol 3-kinase-AKT signaling increases radiosensitivity of K-RAS mutated human tumor cells in vitro by affecting DNA repair. Clin Cancer Res. 2006;12:4119–4126
  17. Dittmann K, Mayer C, Fehrenbacher B, et al. Radiation-induced epidermal growth factor receptor nuclear import is linked to activation of DNA-dependent protein kinase. J Biol Chem. 2005;280:31182–31189
  18. Toulany M, Dittmann K, Kruger M, Baumann M, Rodemann HP. Radioresistance of K-Ras mutated human tumor cells is mediated through EGFR-dependent activation of PI3K–AKT pathway. Radiother Oncol. 2005;76:143–150
  19. Toulany M, Baumann M, Rodemann HP. Stimulated PI3K–AKT signaling mediated through ligand or radiation-induced EGFR depends indirectly, but not directly, on constitutive K-Ras activity. Mol Cancer Res. 2007;5:863–872
  20. Brognard J, Clark AS, Ni Y, Dennis PA. Akt/protein kinase B is constitutively active in non-small cell lung cancer cells and promotes cellular survival and resistance to chemotherapy and radiation. Cancer Res. 2001;61:3986–3997
  21. Golding SE, Morgan RN, Adams BR, Hawkins AJ, Povirk LF, Valerie K. Pro-survival AKT and ERK signaling from EGFR and mutant EGFRvIII enhances DNA double-strand break repair in human glioma cells. Cancer Biol Ther. 2009;8:
  22. Kao GD, Jiang Z, Fernandes AM, Gupta AK, Maity A. Inhibition of phosphatidylinositol-3-OH kinase/Akt signaling impairs DNA repair in glioblastoma cells following ionizing radiation. J Biol Chem. 2007;282:21206–21212
  23. Toulany M, Kehlbach R, Florczak U, et al. Targeting of AKT1 enhances radiation toxicity of human tumor cells by inhibiting DNA-PKcs dependent DNA-double strand break repair. Mol Cancer Ther. 2008;7:1772–1781
  24. Khanna KK, Jackson SP. DNA double-strand breaks: signaling, repair and the cancer connection. Nat Genet. 2001;27:247–254
  25. Iliakis G, Wang H, Perrault AR, et al. Mechanisms of DNA double strand break repair and chromosome aberration formation. Cytogenet Genome Res. 2004;104:14–20
  26. Chan DW, Chen BP, Prithivirajsingh S, et al. Autophosphorylation of the DNA-dependent protein kinase catalytic subunit is required for rejoining of DNA double-strand breaks. Genes Dev. 2002;16:2333–2338
  27. Ding Q, Reddy YV, Wang W, et al. Autophosphorylation of the catalytic subunit of the DNA-dependent protein kinase is required for efficient end processing during DNA double-strand break repair. Mol Cell Biol. 2003;23:5836–5848
  28. Mozdarani H, Nazari E. Frequency of micronuclei in 4–8 cell mouse embryos generated after maternal gamma-irradiation in the presence and in the absence of vitamin C. Radiat Environ Biophys. 2007;46:417–422
  29. Mozdarani H, Gharbali A. Radioprotective effects of cimetidine in mouse bone marrow cells exposed to gamma-rays as assayed by the micronucleus test. Int J Radiat Biol. 1993;64:189–194
  30. Toulany M, Kehlbach R, Florczak U, et al. Targeting of AKT1 enhances radiation toxicity of human tumor cells by inhibiting DNA-PKcs-dependent DNA double-strand break repair. Mol Cancer Ther. 2008;7:1772–1781
  31. Chen BP, Uematsu N, Kobayashi J, et al. Ataxia telangiectasia mutated (ATM) is essential for DNA-PKcs phosphorylations at the Thr-2609 cluster upon DNA double strand break. J Biol Chem. 2007;282:6582–6587
  32. Birkenkamp KU, Coffer PJ. Regulation of cell survival and proliferation by the FOXO (Forkhead box, class O) subfamily of Forkhead transcription factors. Biochem Soc Trans. 2003;31:292–297
  33. Dijkers PF, Birkenkamp KU, Lam EW, et al. FKHR-L1 can act as a critical effector of cell death induced by cytokine withdrawal: protein kinase B-enhanced cell survival through maintenance of mitochondrial integrity. J Cell Biol. 2002;156:531–542
  34. Ju YM, Kim MH, Lee SK, Seo DW, Min YI, Kim JY. Comparative cytotoxicity of low-osmolar nonionic and high-osmolar ionic contrast media to dog gallbladder epithelial cells. Gastrointest Endosc. 2002;55:382–386
  35. Moore DE, Carroll FE, Dutt PL, Reed GW, Holburn GE. Comparison of nonionic and ionic contrast agents in the rabbit lung. Invest Radiol. 1991;26:134–142
  36. Nordby A, Halgunset J, Haugen OA. Cytostatic effects of radiographic contrast media in synchronized cell cultures. Invest Radiol. 1987;22:678–684
  37. Nordby A, Tvedt KE, Halgunset J, Haugen OA. Intracellular penetration and accumulation of radiographic contrast media in the rat kidney. Scanning Microsc. 1990;4:651–664[discussion 664–56]
  38. Parvez Z, Kormano M, Moncada R, Eklund R. Contrast media-induced chromosomal damage in human lymphocyte cultures. Invest Radiol. 1986;21:864–869
  39. Czornak K, Chughtai S, Chrzanowska KH. Mystery of DNA repair: the role of the MRN complex and ATM kinase in DNA damage repair. J Appl Genet. 2008;49:383–396
  40. Nunez ME, Sinues B. Cytogenic effects of diatrizoate and ioxaglate on patients undergoing excretory urography. Invest Radiol. 1990;25:692–697
  41. Parvez Z, Kormano M, Satokari K, Moncada R, Eklund R. Induction of mitotic micronuclei by X-ray contrast media in human peripheral lymphocytes. Mutat Res. 1987;188:233–239
  42. Burma S, Chen BP, Murphy M, Kurimasa A, Chen DJ. ATM phosphorylates histone H2AX in response to DNA double-strand breaks. J Biol Chem. 2001;276:42462–42467
  43. Stiff T, O’Driscoll M, Rief N, Iwabuchi K, Lobrich M, Jeggo PA. ATM and DNA-PK function redundantly to phosphorylate H2AX after exposure to ionizing radiation. Cancer Res. 2004;64:2390–2396

PII: S0167-8140(09)00645-8

doi: 10.1016/j.radonc.2009.11.006

Radiotherapy & Oncology
Volume 94, Issue 1 , Pages 110-116 , January 2010