« Previous
Next »
Radiotherapy & Oncology
Volume 86, Issue 3
, Pages 321-328
, March 2008
In normal human fibroblasts variation in DSB repair capacity cannot be ascribed to radiation-induced changes in the localisation, expression or activity of major NHEJ proteins
References
- . Inverse relationship between epidermal growth factor receptor expression and radiocurability of murine carcinomas. Clin Cancer Res. 1999;5:2884–2890
- . Lack of correlation between DNA-dependent protein kinase activity and tumor cell radiosensitivity. Cancer Res. 1995;55:5200–5202
- . Does variability in normal tissue reactions after radiotherapy have a genetic basis – where and how to look for it?. Radiother Oncol. 2002;64:131–140
- . Prediction of normal tissue radiosensitivity from polymorphisms in candidate genes. Radiother Oncol. 2003;69:127–135
- . Physical interaction between epidermal growth factor receptor and DNA-dependent protein kinase in mammalian cells. J Biol Chem. 1997;273:1568–1573
- . Potential clinical impact of normal-tissue intrinsic radiosensitivity testing. Radiother Oncol. 1997;43:121–131
- . Individualization of radiotherapy dose prescriptions by means of an in vitro radiosensitivity assay. Radiother Oncol. 1998;46:216–218
- . For X-irradiated normal human fibroblasts only half of cell inactivation results from chromosomal damage. Int J Radiat Oncol Biol Phys. 2004;58:445–452
- . Induction and repair of radiation-induced DNA double-strand breaks in human fibroblasts are not affected by terminal differentiation. DNA Repair. 2004;3:113–120
- Ionizing radiation exposure results in up-regulation of Ku70 via a p53/Ataxia telangiectasia-mutated protein-dependent mechanism. J Biol Chem. 2000;275:6651–6656
- . Role of DNA-PK in the cellular response to DNA double-strand breaks. DNA Repair. 2004;3:909–918
- Comparison of DNA repair protein expression and activities between human fibroblast cell lines with different radiosensitivities. Int J Cancer. 2000;85:845–849
- . The contribution of epidermal growth factor receptor (EGFR) signaling pathway to radioresistance in human gliomas: a review of preclinical and correlative clinical data. Int J Radiat Oncol Biol Phys. 2004;58:927–931
- . Characterization of the DNA double strand break repair defect in scid mice. Cancer Res. 1993;53:1244–1248
- Heterogeneous expression of Ku70 in human tissues is associated with morphological and functional alterations of the nucleus. J Pathol. 2002;198:121–130
- . Mechanisms of human DNA repair: an update. Toxicology. 2003;193:3–34
- . Mammalian DNA double-strand break repair protein XRCC4 interacts with DNA ligase IV. Curr Biol. 1997;7:588–598
- . Reparable and non-reparable DNA strand breaks induced by X-irradiation in CHO K1 cells and the radiosensitive mutants xrs1 and xrs5. Int J Radiat Biol. 1993;64:19–26
- Nonhomologous end-joining of site-specific but not of radiation-induced DNA double-strand breaks is reduced in the presence of wild-type p53. Oncogene. 2005;24:1663–1672
- . DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts. Genes Dev. 1994;8:2540–2551
- . Effect of internal beta-irradiation at 0
°C and 37
°C on survival and DNA strand breakage. Int J Radiat Biol. 1987;51:755 - . Relationship between DNA double-strand breaks, cell killing, and fibrosis studied in confluent skin fibroblasts derived from breast cancer patients. Int J Radiat Oncol Biol Phys. 2000;46:481–490
- . Molecular mechanisms of individual radiosensitivity studied in normal diploid human fibroblasts. Toxicology. 2003;193:125–135
- . Inhibition of radiation-induced EGFR nuclear import by C225 (Cetuximab) suppresses DNA-PK activity. Radiother Oncol. 2005;76:157–161
- Radiation-induced epidermal growth factor receptor nuclear import is linked to activation of DNA-dependent protein kinase. J Biol Chem. 2005;280:31182–31189
- . Fibroblast radiosensitivity measured using the comet DNA-damage assay correlates with clonogenic survival parameters. Br J Cancer. 1999;79:1366–1371
- . Hypertonic treatment inhibits radiation-induced nuclear translocation of the Ku proteins G22p1 (Ku70) and Xrcc5 (Ku80) in rat fibroblasts. Radiat Res. 2001;155:320–327
- . Intracellular redistribution of Ku immunoreactivity in response to cell-cell contact and growth modulating components in the medium. J Cell Sci. 1996;109:1937–1946
- . DNA-dependent protein kinase activity is absent in xrs-6 cells: implications for site-specific recombination and DNA double-strand break repair. Proc Natl Acad Sci USA. 1995;92:320–324
- . Expression of DNA-dependent protein kinase holoenzyme upon induction of lymphocyte differentiation and V(D)J recombination. Eur J Biochem. 1996;241:931–940
- Expression of Ku80 in cervical cancer correlates with response to radiotherapy and survival. Am J Clin Oncol. 2003;26:80–85
- . Individual radiosensitivity measured with lymphocytes may be used to predict the risk of fibrosis after radiotherapy for breast cancer. Radiother Oncol. 2003;69:137–144
- . Modulation of radiation response after epidermal growth factor receptor blockade in squamous cell carcinomas: inhibition of damage repair, cell cycle kinetics, and tumor angiogenesis. Clin Cancer Res. 2000;6:2166–2174
- . Evidence for a positive correlation between in vitro radiosensitivity of normal human skin fibroblasts and the occurrence of subcutaneous fibrosis after radiotherapy. Int J Radiat Biol. 1994;66:407–412
- . Ku70/80 gene expression and DNA-dependent protein kinase (DNA-PK) activity do not correlate with double-strand break (dsb) repair capacity and cellular radiosensitivity in normal human fibroblasts. Br J Cancer. 1999;79:1037–1041
- . Overexpression of human Ku70/Ku80 in rat cells resulting in reduced dsb repair capacity with appropriate increase in cell radiosensitivity but with no effect on cell recovery. Radiat Res. 1999;151:532–539
- . Huge differences in cellular radiosensitivity are due to only very small variations in double-strand break repair capacity. Int J Radiat Biol. 2005;81:409–419
- . Radiation-induced chromosome damage in X-ray-sensitive mutants (xrs) of the Chinese hamster ovary cell line. Mutat Res. 1986;166:255–263
- A correlation between residual DNA double-strand breaks and clonogenic measurements of radiosensitivity in fibroblasts from preradiotherapy cervix cancer patients. Int J Radiat Oncol Biol Phys. 1997;39:1137–1144
- . A targeted inhibition of DNA-dependent protein kinase sensitizes breast cancer cells following ionizing radiation. J Pharmacol Exp Ther. 2002;303:753–759
- . Chromosomal localization of the mouse and rat DNA double-strand break repair genes Ku p70 and Ku p80/XRCC5 and their mRNA expression in various mouse tissues. Genomics. 1996;38:38–44
- Differential subcellular localization of DNA-dependent protein kinase components Ku and DNA-PKcs during mitosis. J Cell Sci. 1999;112:4031–4039
- The expression pattern of Ku correlates with tumor radiosensitivity and disease free survival in patients with rectal carcinoma. Cancer. 2002;95:1199–1205
- Expression of Ku70 and DNA-PKcs as prognostic indicators of local control in nasopharyngeal carcinoma. Int J Radiat Oncol Biol Phys. 2005;62:1451–1457
- . Human DNA-Activated protein kinase phosphorylates serines 15 and 37 in the amino-terminal transactivation domain of human p53. Mol Cell Biol. 1992;12:5041–5049
- . Radiation-hypersensitive cancer patients do not manifest protein expression abnormalities in components of the nonhomologous end-joining (NHEJ) pathway. Br J Cancer. 2003;88:1251–1255
- Adenovirus-mediated heat-activated antisense Ku70 expression radiosensitizes tumor cells in vitro and in vivo. Cancer Res. 2003;63:3268–3274
- . Mechanism and regulation of human non-homologous DNA end-joining. Nature. 2003;4:712–720
- . P53 mediates permanent arrest over multiple cell cycles in response to gamma-irradiation. Cancer Res. 1997;57:1171–1179
- . Novel localization of the DNA-PK complex in lipid rafts: a putative role in the signal transduction pathway of the ionizing radiation response. J Biol Chem. 2005;278:22136–22143
- . The modelled benefits of individualizing radiotherapy patients‘dose using cellular radiothsensitivity assays with inherent variability. Radiother Oncol. 1999;50:67–75
- Transfer of Ku86 RNA antisense decreases the radioresistance of human fibroblasts. Cancer Gene Ther. 2000;7:339–346
- A Ku80 fragment with dominant negative activity imparts a radiosensitivity phenotype to CHO-K1 cells. Nucleic Acids Res. 2000;28:4778–4782
- . The double-life of the Ku protein – facing the DNA breaks and the extracellular environment. Cell Cycle. 2005;4:438–441
- . Karp-1 is induced by DNA damage in a p53- and ATM-dependent fashion. Proc Natl Acad Sci USA. 1998;95:7664–7669
- Lack of detectable defect in DNA double-strand break repair and DNA-dependent protein kinase activity in radiosensitive human severe combined immunodeficiency fibroblasts. Eur J Immunol. 1996;26:1118–1122
- . Deficiency in nuclear accumulation of G22p1 and Xrcc5 proteins in hyper-radiosensitive Long–Evans–Cinnamon (LEC) rat cells after x irradiation. Radiat Res. 2002;157:553–561
- . Radiosensitizer effect of Wortmannin in radioresistant bladder tumoral cell lines. Int J Oncol. 2004;24:169–175
- . Defining the minimal domain of Ku80 for interaction with Ku70. J Biol Chem. 1999;272:27259–27265
- . Silencing expression of the catalytic subunit of DNA-dependent protein kinase by small interfering RNA sensitizes human cells for radiation-induced chromosome damage, cell killing and mutation. Cancer Res. 2002;62:6400–6404
- . Mechanisms of DNA double-strand break repair and their potential to induce chromosomal aberrations. Mutagenesis. 2000;15:289–302
- DNA double-strand break repair, DNA-PK, and DNAligases in two human squamous carcinoma cell lines with different radiosensitivity. Int J Radiat Oncol Biol Phys. 1999;43:191–198
- . Cell surface expression of the 70-kD component of Ku, a DNA-binding nuclear autoantigen. J Clin Invest. 1990;86:1301–1305
- Selective inactivation of DNA-dependent protein kinase with antisense oligodeoxynucleotides: consequences for the rejoining of radiation-induced DNA double-strand breaks and radiosensitivity of human cancer cell lines. Cancer Res. 2002;62:6621–6624
- Expression of genes involved in repair of DNA double-strand breaks in normal and tumor tissues. Int J Radiat Oncol Biol Phys. 2001;49:161–167
- . Comparison between pulsed-field gel electrophoresis and the comet assay as predictive assays for radiosensitivity in fibroblasts. Radiat Res. 1998;150:17–22
- Up-regulation of DNA-dependent protein kinase correlates with radiation resistance in oral squamous cell carcinoma. Cancer Sci. 2003;94:894–900
- . DNA-dependent protein kinase content and activity in lung carcinoma cell lines: correlation with intrinsic radiosensitivity. Eur J Cancer. 1999;35:111–116
- . ATM and DNA-PK function redundantly to phosphorylate H2AX after exposure to ionizing radiation. Cancer Res. 2004;64:2390–2396
- Translocation of Ku86/Ku70 to the multiple myeloma cell membrane: functional implications. Exp Hematol. 2002;30:212–220
- EGFR tyrosine kinase domain mutations are detected in histologically normal respiratory epithelium in lung cancer patients. Cancer Res. 2005;65:7568–7572
- The 86-kD subunit of Ku autoantigens mediates homotypic and heterotypic adhesion of multiple myeloma cells. J Clin Invest. 1998;101:1379–1388
- Relationship between antiapoptotic molecules and metastatic potency and the involvement of DNA-dependent protein kinase in the chemosensitization of metastatic human cancer cells by epidermal growth factor receptor blockade. J Pharmacol Exp Ther. 2004;311:1062–1070
- Decreased DNA-PK activity in human cancer cells exhibiting hypersensitivity to low-dose irradiation. Br J Cancer. 2000;83:514–518
- . Regulation and mechanisms of mammalian double-strand break repair. Oncogene. 2003;22:5792–5812
- . Expression of Ku70 correlates with survival in carcinoma of the cervix. Br J Cancer. 2000;83:1702–1706
- . Cellular radiosensitivity and DNA damage in primary human fibroblasts. Int J Radiat Oncol Biol Phys. 1994;30:625–633
- DNA-dependent protein kinase activity correlates with Ku70 expression and radiation sensitivity in esophageal cancer cell lines. Clin Cancer Res. 2000;6:1073–1078
PII: S0167-8140(07)00652-4
doi: 10.1016/j.radonc.2007.11.035
© 2007 Elsevier Ireland Ltd. All rights reserved.
« Previous
Next »
Radiotherapy & Oncology
Volume 86, Issue 3
, Pages 321-328
, March 2008
