Radiotherapy & Oncology
Volume 86, Issue 3 , Pages 354-360 , March 2008

Tissue oxygenation in a murine SCC VII tumor after X-ray irradiation as determined by EPR spectroscopy

  • Hirotada Fujii

      Affiliations

    • School of Health Sciences and
  • ,
  • Koh-ichi Sakata

      Affiliations

    • Department of Radiology, Sapporo Medical University, Sapporo, Japan
  • ,
  • Yoshihiro Katsumata

      Affiliations

    • Graduate Program of Human Sensing and Functional Sensor Engineering, and
  • ,
  • Rikiya Sato

      Affiliations

    • Graduate Program of Human Sensing and Functional Sensor Engineering, and
  • ,
  • Makoto Kinouchi

      Affiliations

    • Department of Bio-System Engineering, Yamagata University, Yonezawa, Japan
  • ,
  • Masanori Someya

      Affiliations

    • Department of Radiology, Sapporo Medical University, Sapporo, Japan
  • ,
  • Shin-ichiro Masunaga

      Affiliations

    • Particle Radiation Oncology Research Center, Research Reactor Institute, Kyoto University, Osaka, Japan
  • ,
  • Masato Hareyama

      Affiliations

    • Department of Radiology, Sapporo Medical University, Sapporo, Japan
  • ,
  • Harold M. Swartz

      Affiliations

    • EPR Center for Viable Systems, Department of Radiology, Dartmouth Medical School, Hanover, NH, USA
  • ,
  • Hiroshi Hirata

      Affiliations

    • Graduate Program of Human Sensing and Functional Sensor Engineering, and
    • Department of Electrical Engineering, Yamagata University, Yonezawa, Japan
    • Corresponding Author InformationCorresponding author. Hiroshi Hirata, Department of Electrical Engineering, Yamagata University, Yonezawa, Yamagata 992-8510, Japan.

Received 3 July 2007 ,Revised 19 November 2007 ,Accepted 19 November 2007.

References 

  1. Ling CC, Humm J, Larson S, et al. Towards multidimensional radiotherapy (MD-CRT): biological imaging and biological conformality. Int J Radiat Oncol Biol Phys. 2000;47:551–560
  2. Hall EJ. Oxygen effect and reoxygenation. In:  Hall EJ editors. Radiobiology for the radiologist. 6th ed.. Philadelphia: Lippincott Williams & Wilkins; 2006;p. 85–105
  3. Brizel DM, Sibley GS, Prosnitz LR, et al. Tumor hypoxia adversely affects the prognosis of carcinoma of the head and neck. Int J Radiat Oncol Biol Phys. 1997;38:285–289
  4. Teicher BA. Hypoxia and drug resistance. Cancer Metast Rev. 1994;13:139–168
  5. Hockel M, Schlenger K, Aral B, et al. Association between tumor hypoxia and malignant progression in advanced cancer of the uterine cervix. Cancer Res. 1996;56:4509–4515
  6. Nordsmark M, Bentzen SM, Rudat V, et al. Prognostic value of tumor oxygenation in 397 head and neck tumors after primary radiation therapy. An international multi-center study. Radiother Oncol. 2005;77:18–24
  7. Nordsmark M, Overgaard J. A confirmatory prognostic study on oxygenation status and loco-regional control in advanced head and neck squamous cell carcinoma treated by radiation therapy. Radiother Oncol. 2000;57:39–43
  8. Nordsmark M, Overgaard M, Overgaard J. Pretreatment oxygenation predicts radiation response in advanced squamous cell carcinoma of the head and neck. Radiother Oncol. 1996;41:31–39
  9. Overgaard J. Sensitization of hypoxic tumour cells – clinical experience. Int J Radiat Biol. 1989;56:801–811
  10. Gallez B, Baudelet C, Jordan BF. Assessment of tumor oxygenation by electron paramagnetic resonance: principles and applications. NMR Biomed. 2004;17:240–262
  11. Swartz HM, Boyer S, Brown D, et al. The use of EPR for the measurement of the concentration of oxygen in vivo in tissues under physiologically pertinent conditions and concentrations. Adv Exp Med Biol. 1992;317:221–228
  12. Liu KJ, Gast P, Moussavi M, et al. Lithium phthalocyanine: a probe for electron paramagnetic resonance oximetry in viable biological systems. Proc Natl Acad Sci USA. 1993;90:5438–5442
  13. Pandian RP, Parinandi NL, Ilangovan G, et al. Novel particulate spin probe for targeted determination of oxygen in cells and tissues. Free Radic Biol Med. 2003;35:1138–1148
  14. Khan N, Williams BB, Swartz HM. Clinical applications of in vivo EPR: rationale and initial results. Appl Magn Reson. 2006;30:185–199
  15. Swartz HM, Khan N, Buckey J, et al. Clinical applications of EPR: overview and perspectives. NMR Biomed. 2004;17:335–351
  16. Kitakabu Y, Shibamoto Y, Sasai K, et al. Variations of the hypoxic fraction in the SCC VII tumors after single dose and during fractionated radiation therapy: assessment without anesthesia or physical restraint of mice. Int J Radiat Oncol Biol Phys. 1991;20:709–714
  17. Hirata H, Walczak T, Swartz HM. Electronically tunable surface-coil-type resonator for L-band EPR spectroscopy. J Magn Reson. 2000;142:159–167
  18. Fujii H, Koscielniak J, Berliner LJ. Determination and characterization of nitric oxide generation in mice by in vivo L-band EPR spectroscopy. Magn Reson Med. 1997;38:565–568
  19. Kim IH, Brown JM. Reoxygenation and rehypoxiation in the SCCVII mouse tumor. Int J Radiat Oncol Biol Phys. 1994;29:493–497
  20. Olive PL. Radiation-induced reoxygenation in the SCCVII murine tumour: evidence for a decrease in oxygen consumption and an increase in tumour perfusion. Radiother Oncol. 1994;32:37–46
  21. Secomb TW, Hsu R, Dewhirst MW. Synergistic effects of hyperoxic gas breathing and reduced oxygen consumption on tumor oxygenation: a theoretical model. Int J Radiat Oncol Biol Phys. 2004;59:572–578
  22. Fenton BM, Lord EM, Paoni SF. Effects of radiation on tumor intravascular oxygenation, vascular configuration, development of hypoxia, and clonogenic survival. Radiat Res. 2001;155:360–368
  23. Ljungkvist AS, Bussink J, Kaanders JH, et al. Dynamics of hypoxia, proliferation and apoptosis after irradiation in a murine tumor model. Radiat Res. 2006;165:326–336
  24. Nordsmark M, Bentzen SM, Rudat V, et al. Prognostic value of tumor oxygenation in 397 head and neck tumors after primary radiation therapy: An international multi-center study. Radiother Oncol. 2005;77:18–24
  25. Bratasz A, Pandian RP, Deng Y, et al. In vivo imaging of changes in tumor oxygenation during growth and after treatment. Magn Reson Med. 2007;57:950–959
  26. Crokart N, Jordan BF, Baudelet C, et al. Early reoxygenation in tumors after irradiation: determining factors and consequences for radiotherapy regimens using daily multiple fractions. Int J Radiat Oncol Biol Phys. 2005;63:901–910
  27. O’Hara JA, Goda F, Demidenko E, et al. Effect on regrowth delay in a murine tumor of scheduling split-dose irradiation based on direct pO2 measurements by electron paramagnetic resonance oximetry. Radiat Res. 1998;150:549–556
  28. Evans SM, Koch CJ. Prognostic significance of tumor oxygenation in humans. Cancer Lett. 2003;195:1–16
  29. Grinberg OY, Smirnov AI, Swartz HM. High spatial resolution multi-site EPR oximetry. The use of convolution-based fitting method. J Magn Reson. 2001;152:247–258

PII: S0167-8140(07)00606-8

doi: 10.1016/j.radonc.2007.11.020

Radiotherapy & Oncology
Volume 86, Issue 3 , Pages 354-360 , March 2008