Radiotherapy & Oncology
Volume 76, Issue 2 , Pages 162-167 , August 2005

Decreased repopulation as well as increased reoxygenation contribute to the improvement in local control after targeting of the EGFR by C225 during fractionated irradiation

  • Mechthild Krause

      Affiliations

    • Department of Radiation Oncology, and
  • ,
  • Gernot Ostermann

      Affiliations

    • Department of Radiation Oncology, and
  • ,
  • Cordula Petersen

      Affiliations

    • Department of Radiation Oncology, and
  • ,
  • Ala Yaromina

      Affiliations

    • Department of Radiation Oncology, and
  • ,
  • Franziska Hessel

      Affiliations

    • Department of Radiation Oncology, and
  • ,
  • Andreas Harstrick

      Affiliations

    • Merck KG aA, Darmstadt, Germany
  • ,
  • Albert J van der Kogel

      Affiliations

    • Department of Radiation Oncology, University Medical Centre Nijmegen, The Netherlands
  • ,
  • Howard D Thames

      Affiliations

    • MD Andersen Cancer Center, Houston, TX, USA
  • ,
  • Michael Baumann

      Affiliations

    • Department of Radiation Oncology, and
    • Experimental Centre, Medical Faculty Carl Gustav Carus, University of Technology Dresden, Germany
    • Corresponding Author InformationCorresponding author. M. Baumann, Department of Radiation Oncology, Medical Faculty Carl Gustav Carus, University of Technology Dresden, Fetscherstr. 74, 01307 Dresden, Germany.

Received 3 May 2005 ,Revised 13 May 2005 ,Accepted 18 June 2005.

References 

  1. Baumann M, Dubois W, Suit HD. Response of human squamous cell carcinoma xenografts of different sizes to irradiation: relationship of clonogenic cells, cellular radiation sensitivity in vivo, and tumor rescuing units. Radiat Res. 1990;123:325–330
  2. Baumann M, Krause M. Targeting the epidermal growth factor receptor in radiotherapy: radiobiological mechanisms, preclinical and clinical results. Radiother Oncol. 2004;72:257–266
  3. Baumann M, Krause M, Zips D, et al. Selective inhibition of the epidermal growth factor tyrosine kinase by BIBX1382BS improves growth delay but not local control after fractionated irradiation in human FaDu squamous cell carcinoma in nude mice. Int J Radiat Biol. 2003;79:547–559
  4. Baumann M, Liertz C, Baisch H, Wiegel T, Lorenzen J, Arps H. Impact of overall treatment time of fractionated irradiation on local control of human FaDu squamous cell carcinoma in nude mice. Radiother Oncol. 1994;32:137–143
  5. Beck-Bornholdt HP. Should tumors be clamped in radiobiological fractionation experiments?. Int J Radiat Oncol Biol Phys. 1991;21:675–682
  6. Bentzen SM, Atasoy BM, Daley FM, et al. Epidermal growth factor receptor expression in pre-treatment biopsies from head and neck squamous cell carcinoma as a predictive factor for a benefit from accelerated radiation therapy in a randomized controlled trial. J Clin Oncol; in press.
  7. Bonner JA, Harari PM, Giralt JL. Cetuximab (Erbitux TM) prolongs survival in patients with locally advanced squamous cell carcinoma of the head and neck: a phase III study of high dose radiation therapy with and without cetuximab. Proc Am Soc Clin Oncol. 2004;5507;[abstract]
  8. In:  Davison AC,  Hinkley DV editor. Bootstrap methods and their application. Cambridge: Cambridge University press; 1997;
  9. Efron B, Tibshirani R. Bootstrap measures for standard errors, confidence intervals, and other measures of statistical accuracy. Stat Sci. 1986;1:54–77
  10. Eriksen JG, Steiniche T, Overgaard J. The influence of epidermal growth factor receptor and tumor differentiation on the response to accelerated radiotherapy of squamous cell carcinomas of the head and neck in the randomized DAHANCA 6 and 7 study. Radiother Oncol. 2005;74:93–100
  11. Hessel F, Petersen C, Zips D, et al. Impact of increased cell loss on the repopulation rate during fractionated irradiation in human FaDu squamous cell carcinoma growing in nude mice. Int J Radiat Biol. 2003;79:479–486
  12. Huang SM, Bock JM, Harari PM. Epidermal growth factor receptor blockade with C225 modulates proliferation, apoptosis, and radiosensitivity in squamous cell carcinomas of the head and neck. Cancer Res. 1999;59:1935–1940
  13. Huang SM, Harari PM. Modulation of radiation response after epidermal growth factor receptor blockade in squamous cell carcinomas: inhibition of damage repair, cell cycle kinetics, and tumor angiogenesis. Clin Cancer Res. 2000;6:2166–2174
  14. Huang SM, Li J, Armstrong EA, Harari PM. Modulation of radiation response and tumor-induced angiogenesis after epidermal growth factor receptor inhibition by ZD1839 (Iressa). Cancer Res. 2002;62:4300–4306
  15. Krause M, Hessel F, Zips D, Hilberg F, Baumann M. Adjuvant inhibition of the epidermal growth factor receptor after fractionated irradiation of FaDu human squamous cell carcinoma. Radiother Oncol. 2004;72:95–101
  16. Krause M, Schutze C, Petersen C, et al. Different classes of EGFR inhibitors may have different potential to improve local tumour control after fractionated irradiation: a study on C225 in FaDu hSCC. Radiother Oncol. 2005;74:109–115
  17. Laderoute KR, Grant TD, Murphy BJ, Sutherland RM. Enhanced epidermal growth factor receptor synthesis in human squamous carcinoma cells exposed to low levels of oxygen. Int J Cancer. 1992;52:428–432
  18. Lammering G. Molecular predictor and promising target: will EGFR now become a star in radiotherapy?. Radiother Oncol. 2005;74:89–91
  19. Lammering G, Hewit TH, Holmes M, et al. Inhibition of the type III epidermal growth factor receptor variant mutant receptor by dominant-negative EGFR-CD533 enhances malignant glioma cell radiosensitivity. Clin Cancer Res. 2004;10:6732–6743
  20. Leith JT, Harrigan P, Padfield G, Faulkner L, Michelson S. Modification of the growth rates and hypoxic fractions of xenografted A431 tumors by sialoadenectomy or exogenously supplied epidermal growth factor. Cancer Res. 1991;51:4111–4113
  21. Lynch TJ, Bell DW, Sordella R, et al. Activating mutations in the epidermal growth factor receptor underlying responsiveness of non-small-cell lung cancer to gefitinib. N Engl J Med. 2004;350:2129–2139
  22. Milas L, Mason K, Hunter N, et al. In vivo enhancement of tumor radioresponse by C225 antiepidermal growth factor receptor antibody. Clin Cancer Res. 2000;6:701–708
  23. Milas L, Mason KA, Ang KK. Epidermal growth factor receptor and its inhibition in radiotherapy: in vivo findings. Int J Radiat Biol. 2003;79:539–545
  24. Nishi H, Nishi KH, Johnson AC. Early growth response-1 gene mediates up-regulation of epidermal growth factor receptor expression during hypoxia. Cancer Res. 2002;62:827–834
  25. Paez JG, Janne PA, Lee JC, et al. EGFR mutations in lung cancer: correlation with clinical response to gefitinib therapy. Science. 2004;
  26. Petersen C, Zips D, Krause M, et al. Repopulation of FaDu human squamous cell carcinoma during fractionated radiotherapy correlates with reoxygenation. Int J Radiat Oncol Biol Phys. 2001;51:483–493
  27. Schmidt-Ullrich RK, Mikkelsen RB, Dent P, et al. Radiation-induced proliferation of the human A431 squamous carcinoma cells is dependent on EGFR tyrosine phosphorylation. Oncogene. 1997;15:1191–1197
  28. In:  Steel GG editors. Growth kinetics of tumours: cell population kinetics in relation to the growth and treatment of cancer. Oxford: Clarendon Press; 1977;
  29. Toulany M, Dittmann K, Baumann M, Rodemann HP. Radiosensitization of ras-mutated human tumor cells in vitro by the specific EGF receptor antagonist BIBX1382BS. Radiother Oncol. 2005;74:117–129
  30. van Laarhoven HW, Bussink J, Lok J, Punt CJ, Heerschap A, van Der Kogel AJ. Effects of nicotinamide and carbogen in different murine colon carcinomas: immunohistochemical analysis of vascular architecture and microenvironmental parameters. Int J Radiat Oncol Biol Phys. 2004;60:310–321
  31. Walker AM, Suit HD. Assessment of local tumor control using censored tumor response data. Int J Radiat Oncol Biol Phys. 1983;9:383–386
  32. Zietman AL, Suit HD, Ramsay JR, Silobrcic V, Sedlacek RS. Quantitative studies on the transplantability of murine and human tumors into the brain and subcutaneous tissues of NCr/Sed nude mice. Cancer Res. 1988;48:6510–6516

PII: S0167-8140(05)00247-1

doi: 10.1016/j.radonc.2005.06.032

Radiotherapy & Oncology
Volume 76, Issue 2 , Pages 162-167 , August 2005